Journal: The EMBO Journal

Article Title: The global phosphorylation landscape of mouse oocytes during meiotic maturation

doi: 10.1038/s44318-024-00222-1

Figure Lengend Snippet: Reagents and tools table

Article Snippet: MP7 , MCE , Cat# HY14440.

Techniques: Virus, Isolation, Reverse Transcription, Purification, SYBR Green Assay, Western Blot, Silver Staining, Sequencing, Over Expression, Recombinant, Plasmid Preparation, Mutagenesis, Software

Journal: Autophagy

Article Title: Stimulating the autophagic-lysosomal axis enhances host defense against fungal infection in a zebrafish model of invasive Aspergillosis.

doi: 10.1080/15548627.2022.2090727

Figure Lengend Snippet: Figure 5. Stimulating the (auto)phagolysosomal axis increases the host survival against IA. (a, b and c) Survival curves of zebrafish embryos infected with A. fumigatus ∆Ku80 and exposed via waterborne treatment to 10 nM of torin 1 (a), 10 μM of AR-12 (b), 100 μM or carbamazepine (c), or DMSO as a vehicle control at the same v:v. (a, b, and c) infections were performed together but showed in split graphs for visualization purposes. (d) Survival curve of dram1 mutants or non- mutant siblings infected with A. fumigatus ∆Ku80. (e) Survival curve of zebrafish embryos overexpressing Dram1 after injection of 100 pg of dram1 mRNA or their control infected with A. fumigatus ∆Ku80. (e) Confocal image demonstrating colocalization (arrowheads) of mCherry-Dram1 with Alexa Fluor™ NHS 647-labeled A. fumigatus conidia (Af647) inside a phagocyte in the zebrafish hindbrain shortly after infection. All survival curves are representative of at least 3 independent biological replicates. The hazard ratio (HR) indicated is calculated vs. the control condition using the logrank method. Significance in the curve comparison is calculated using Log-rank (Mantel-Cox test): ns non-significant; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: Dexamethasone (Sigma-Aldrich, D1756-25 MG), FK506 (InvivoGen, tlrl-fk5), rapamycin (Sigma-Aldrich, 44,532-U), cyclosporin A, metronidazole (Sigma-Aldrich, M1547-5 G), AR-12 (MedChem Express, HY-10547_5 mg), carbamazepine (MedChem Express, HY-B0246-100 mg), torin 1 (MedChem Express, HY-13003_5 mg), and voriconazole (Sigma-Aldrich, PZ0005-5 MG) were resuspended at 10 mg/ml in DMSO (Sigma-Aldrich, 154,938) and aliquoted in 100 μl stock concentrations at the recommended temperature for long-term storage for each drug.

Techniques: Infection, Control, Mutagenesis, Injection, Labeling, Comparison

Journal: Oncology Letters

Article Title: PDK1-WNK1 signaling is affected by HBx and involved in the viability and metastasis of hepatic cells

doi: 10.3892/ol.2018.8001

Figure Lengend Snippet: PDK1 and WNK1 are activated in HBx-transfected hepatic cells. (A) The expression of PDK1/p-PDK1 and WNK1/p-WNK1 in LO2 and SK-Hep1 cells following HBx-gene transfection was evaluated using western blot analysis. (B) HBx expression in LO2-HBx and SK-Hep1-HBx cells determined using the reverse transcription-quantitative polymerase chain reaction. HepG2.2.15 was used as positive control. *P<0.05. Western blot analysis of the expression of PDK1/p-PDK1 and WNK1/p-WNK1 in response to a PDK1 inhibitor in (C) LO2/LO2-HBx and (D) SK-Hep1/Hep1-HBx cells. PDK1, 3-phosphoinositide-dependent protein kinase-1; WNK1, with-no-lysine (K) kinase 1; p, phospho; C, control.

Article Snippet: Cells were treated with 0, 5 or 20 μM PDK1 inhibitor II (cat. no. 521276; Merck KGaA, Darmstadt, Germany) in a humidified incubator (37°C, 5% CO 2 ).

Techniques: Transfection, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Positive Control, Control

Journal: Oncology Letters

Article Title: PDK1-WNK1 signaling is affected by HBx and involved in the viability and metastasis of hepatic cells

doi: 10.3892/ol.2018.8001

Figure Lengend Snippet: HBx attenuates the effect of a PDK1 inhibitor on the viability of hepatic cells. (A) Viability of LO2/LO2-HBx cells in response to 0, 5 or 20 µM PDK1 inhibitor. (B) Viability of SK-Hep1/SK-Hep1-HBx cells in response to 0, 5 or 20 µM PDK1 inhibitor using a Cell Counting kit-8. *P<0.05; **P<0.01 vs. C. (C) Viability of LO2/LO2-HBx cells in response to 20 µM PDK1 inhibitor for 0, 24, 48 or 72 h. (D) Viability of SK-Hep1/SK-Hep1-HBx cells in response to 20 µM PDK1 inhibitor for 0, 24, 48 or 72 h. *P<0.05; **P<0.01 vs. HBx-non-expressing group. Experiments were performed in triplicate at least three times. PDK1, 3-phosphoinositide-dependent protein kinase-1; WNK1, with-no-lysine (K) kinase 1; C, control.

Article Snippet: Cells were treated with 0, 5 or 20 μM PDK1 inhibitor II (cat. no. 521276; Merck KGaA, Darmstadt, Germany) in a humidified incubator (37°C, 5% CO 2 ).

Techniques: Cell Counting, Expressing, Control

Journal: Oncology Letters

Article Title: PDK1-WNK1 signaling is affected by HBx and involved in the viability and metastasis of hepatic cells

doi: 10.3892/ol.2018.8001

Figure Lengend Snippet: PDK1 inhibitor suppresses the migration of hepatic cells. (A) Representative images of migrated LO2/LO2-HBx cells in response to 0, 5 or 20 µM PDK1 inhibitor (scale bar, 100 µm). (B) Cell migration rate of LO2/LO2-HBx cells in response to 0, 5 or 20 µM PDK1 inhibitor. (C) Representative images of migrated SK-Hep1/SK-Hep1-HBx cells in response to 0, 5 or 20 µM PDK1 inhibitor (scale bar, 100 µm). (D) Cell migration rate of SK-Hep1/SK-Hep1-HBx cells in response to 0, 5 or 20 µM PDK1 inhibitor. *P<0.05; **P<0.01 vs. C. PDK1, 3-phosphoinositide-dependent protein kinase-1; C, control.

Article Snippet: Cells were treated with 0, 5 or 20 μM PDK1 inhibitor II (cat. no. 521276; Merck KGaA, Darmstadt, Germany) in a humidified incubator (37°C, 5% CO 2 ).

Techniques: Migration, Control

Journal: Oncology Letters

Article Title: PDK1-WNK1 signaling is affected by HBx and involved in the viability and metastasis of hepatic cells

doi: 10.3892/ol.2018.8001

Figure Lengend Snippet: p-PDK1 is upregulated in HCC tissues. (A) Expression levels of p-PDK1 in 21 primary HCC and paired normal liver tissue specimens. (B) Western blot analysis of the expression of p-PDK1 in HCC and paired non-tumor samples. *P<0.05. HCC, hepatocellular carcinoma; PDK1, 3-phosphoinositide-dependent protein kinase-1; p, phospho; C, control; T, tumor; NT, non-tumor.

Article Snippet: Cells were treated with 0, 5 or 20 μM PDK1 inhibitor II (cat. no. 521276; Merck KGaA, Darmstadt, Germany) in a humidified incubator (37°C, 5% CO 2 ).

Techniques: Expressing, Western Blot, Control